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. Author manuscript; available in PMC: 2015 Dec 10.
Published in final edited form as: FEMS Microbiol Lett. 2014 Oct 31;361(2):107–114. doi: 10.1111/1574-6968.12619

Fig. 2.

Fig. 2

T3SS1 and T3SS2 effectors are produced and secreted at similar levels by POR and CAB strains. (a) Secretion of T3SS1 effectors, VopQ and VopS, from T3SS1-induced strains grown in DMEM for 3 h at 37 °C. (b) Secretion of T3SS2 effectors, VopA and VopL, from T3SS2-induced strains grown in MLB with 0.05% bile salts for 3 h at 37 °C. Proteins from bacterial lysates or TCA-precipitated media detected by immunoblot analysis using anti-VopQ, anti-VopS, anti-VopA, and anti-VopL antibodies. Asterisk represents nonspecific band recognized by antibody and LC is abbreviation for loading control.