Fig. 5.
ExsA mutant exhibits better invasion than T3SS structural mutant. HeLa cell invasion with POR and CAB strains. HeLa cells were incubated with bacteria at MOI of 10 for 2 h in 1 mL total volume prior to inoculation with 100 µg mL−1 gentamicin to kill extracellular bacteria. Intracellular bacteria quantified 3 h after gentamicin treatment as colony forming units per milliliter (CFU mL−1). Error bars show standard deviation (n = 3).