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. Author manuscript; available in PMC: 2015 Dec 10.
Published in final edited form as: Receptors Clin Investig. 2015 Nov 4;2(4):1041.

Fig 3. Effect of PEG-rMuIL-10 dosing on intratumoral CD8+ T cell infiltration and function.

Fig 3

ELISPOTs (R&D Systems) were generated by magnetic (Miltenyi) bead isolation of 1000 CD8+ T cells from PBMC or mechanically disrupted and enzyme digested CT26 (ATCC) tumors. CD8+ T cells were exposed for 24 hrs to no secondary stimulus, (w/o), 1 µg/mL soluble anti-CD3 (eBiosciences), 100 CT26 squamous tumor or 4T1 (ATCC) (as negative control) tumor cells. Spots were quantified with ImmunoSpot Software.