Figure 2.

Differences between spheroids prepared in a HA gel and on a nonadherent dish. (A): Microscopic images of adipose-derived stem/stromal cell (ASC) spheroids that were prepared by 48-hour 3D culture in 4% HA gel or on a nonadherent dish. Scale bars = 100 μm. (B): Representative histograms of ASC spheroid diameter prepared in 4% HA gel or on a nonadherent dish. Cell aggregates with a diameter >20 μm were regarded as spheroids (blue). In the HA gel the spheroid diameter showed a unimodal distribution and was relatively small. By contrast, the diameter showed bimodal distribution on the nonadhesive dish, and a substantial number of ASC spheroids were >100 μm in diameter. Abbreviations: 3D, three-dimensional; h, hour(s); HA, hyaluronic acid.