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. 2015 Oct 22;4(12):1511–1522. doi: 10.5966/sctm.2015-0037

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Figure 3. — Microarray analysis of human adipose-derived stem/stromal cells (ASCs) prepared by monolayer culture versus ASC spheroids by three-dimensional culture in hyaluronic acid gel. ASC spheroids showed increased gene expression of some angiogenic growth factors (VEGFA, VEGFB, HGF, PDGFA, PDGFC), anti-inflammatory factors (IL1RN, IL11, etc.), and pluripotent markers (NANOG, OCT3/4, STAT3, etc.) compared with monolayer-cultured ASCs. In contrast, gene expression related to the mitotic cell cycle decreased thoroughly in ASC spheroids.