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. 2015 Oct 22;4(12):1511–1522. doi: 10.5966/sctm.2015-0037

Figure 4.

Figure 4.

Immunostaining of adipose-derived stem/stromal cell (ASC) spheroids prepared in HA gel. (A): Microscopically, spheroids were consistently orbicular in shape and had a smooth surface. Scale bar = 15 μm. (B): Immunocyto-/histochemistry for CD90. CD90 was expressed by almost all cells in monolayer, nonadherent, and HA spheroids. Scale bar = 30 μm. (C): Representative immunostaining of cryosections. ASC spheroids expressed pluripotent stem cell markers such as NANOG, OCT3/4, and SOX-2. Scale bar = 30 μm. (D): Immunocyto-/histochemistry for SSEA-3. SSEA-3 was most highly expressed by HA spheroids compared with monolayer-cultured ASCs and nonadherent culture spheroids. Scale bar = 50 μm. (E): Some of the ASC spheroids were positive for SSEA-3, a pluripotent stem cell (muse cell) marker. SSEA-3(+)-rich spheroids constituted approximately 40% of ASC spheroids. Scale bars = 30 μm. Abbreviation: HA, hyaluronic acid.