Figure 7.

Histological assessment of vasculature and contribution of administered adipose-derived stem/stromal cells (ASCs). (A): The number of capillaries were counted using microsections stained with isolectin. Although the ASC spheroid group showed the highest capillary density at 7 days, this change was no longer significant at 4 weeks. (B): Immunostained section of spheroid-treated sample at 1 week. The human Golgi-positive cells (injected ASCs) were detected mainly in the degenerated adipose area, which showed less perilipin expression. Bar = 40 μm. (C): Immunostained section of spheroid-treated sample at 4 weeks. Some of the h-Golgi(+) cells (arrowheads) expressed isolectin, indicating that they were incorporated into the repaired adipose tissue as vascular endothelial cells. Scale bars = 50 μm. ∗, p < .05. Abbreviations: d, days; h-Golgi(+), human Golgi-positive; PBS, phosphate-buffered saline.