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. 2015 Dec 10;10(12):e0144462. doi: 10.1371/journal.pone.0144462

Fig 6. The regulatory pathways of curcumin on adiponectin expression.

Fig 6

(A) A549 cells were treated with various PI3K/AKT and MAP kinase pathway inhibitors {(PI3K (LY294002; 10 μM), AKT (API-59; 3 μM), MAPK inhibitors [PD98059 (10 μM), SB203580 (10 μM), and SP600125 (10 μM) for ERK, p38–MAPK, and JNK, respectively]} for 1 h and later with curcumin (50 μM) for 24 h. Adiponectin expression was analyzed by western blotting. (B) AKT expression was analyzed by western blotting with different concentrations of recombination adiponectin. (C) The activity of p65/p50 of A549 cells was analyzed by EMSA after treatment with recombinant adiponectin or AKT inhibitor (API-59; 3 μM), respectively.