Fig 1. Characterization of the single- and multicopy gfp-reporter.

(A) Genetic organization of the ColIb locus in S. Tm^wt and (B) the singlecopy reporter S. Tm p2^cib::sfgfp. (C) Schematic view of the multicopy reporter p^{Pcib gfp}. (D) S. Tm p2^cib::sfgfp and (E) S. Tm p^{Pcib gfp} were grown in LB (grey), or in LB supplemented with 0.25μg/ml MitC (red), 100μM DTPA (blue) or with both supplements (purple). Bacteria were analyzed for sfGFP or GFP -signal intensities by flow cytometry, respectively. S. Tm^wt lacking the reporter was used as negative control and for calculating the fraction (%) of GFP⁺ bacteria (green).