| SB300 |
S. Tmwt
|
S. Tm SL1344, SmR
|
[24] |
| M1400 |
S. Tm p2cib-HA
|
S. Tm SL1344 cib-HA-aphT, SmR, KanR
|
This study |
| SJB15-2 |
S. Tm p2cib::sfgfp
|
S. Tm SL1344 cib imm::sfgfp-aphT SmR, KanR
|
This study |
|
E. coli strains
|
|
|
|
| DH5α |
Ec
DH5α
|
F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk
-, mk
+) phoA supE44 thi-1 gyrA96 relA1 λ-
|
Invitrogen |
| MG1655 |
Ec
MG1655
|
E. coli K-12 strain MG1655, F- lambda- ilvG- rfb-50 rph-1; RifR, SmR
|
[25, 26] |
|
Plasmids
|
|
|
|
| pWKS30 |
|
Low-copy vector; pSC101-based replicon; ampicillin-resistance marker |
[27] |
| pKD46 |
|
Temperature sensitive replication (repA101ts); encodes lambda Red genes (exo, bet, gam); native terminator (tL3) after exo gene; arabinose-inducible promoter for expression (ParaB); encodes araC for repression of ParaB promoter; ampicillin resistance |
[28] |
| pWRG7 |
|
High-copy vector, colE1-replicon; carries sfgfp aphT flanked by FRT sequences; ampicillin resistance |
This study |
| pSU315 |
|
Low-copy vector; R6K gamma replicon |
[20] |
| pSJB17 |
pcontrol
|
pBR322-derivative, ampicillin resistance |
This study |
| pSJB16 |
pPcib
|
pBR322-derivative, Pcib, ampicillin resistance |
This study |
| pM968 |
pgfp
|
pBR322-derivative, promoterless, gfpmut2; ampicillin resistance |
[29] |
| pM1437 |
pPcib gfp
|
pBR322-derivative, Pcib, gfpmut2; ampicillin resistance |
[10] |
| pM979 |
pPrpsm gfp
|
pBR322-derivative, PrpsM, (ribosomal rpsM promoter, constitutive), gfpmut2, ampicillin resistance |
[29] |
| pM974 |
pPsicA gfp
|
pBR322-derivative, PsicA (locus in Salmonella pathogenicity island 1), gfpmut2, ampicillin resistance |
[29, 30] |
