Fig 4. Lineweaver-Burk plot of the inhibitory effect of Ro 31–8220 on TEA uptake in HEK-OCT1 cells.

HEK-OCT1 cells were incubated with various concentrations of TEA (from 10 to 1040 μM) for 5 min at 37°C, in the absence or presence of 0.5 μM Ro 31–8220. After washing with ice- cold PBS, intracellular accumulation of TEA was determined by scintillation counting and normalized to total protein content. Data shown are the means ± SEM of four independent experiments.