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. Author manuscript; available in PMC: 2015 Dec 10.
Published in final edited form as: Nano Lett. 2015 Jul 27;15(8):5025–5032. doi: 10.1021/acs.nanolett.5b01047

Figure 4.

Figure 4

Quantification of MT1-MMP QD/FRET ratio and QD intensity in MDA-MB-231 breast cancer cells. (a) Representative images of cells and nanosensors with (left) or without (right) GM pretreatment. QD/FRET ratio images (top panels) were overlaid with cell edges (white lines). QD intensity images (middle panels) were overlaid with detected QD clusters (red lines) and cell edges (white lines). DIC images are shown on the bottom row. (b) Pixel-wise histogram of QD/FRET ratio (top) and QD intensity (bottom) within the detected nanosensor clusters of the cell. (c) Comparison of the QD/FRET ratio, QD intensity, and cell sizes between the cells with (n = 20) or without GM pretreatment (n = 22). * indicates statistically significant differences, p < 0.002. Error bar: SEM (d) Comparison of the QD intensity in cells before and after BCG treatment. The intensity values were normalized to those in the same cell before BCG treatment, so that the QD intensity before BCG treatment is 1.