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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Leukemia. 2015 Jul 23;29(12):2328–2337. doi: 10.1038/leu.2015.196

Figure 4. The effects of HS-5 co-culture are confirmed in CD34+ cells from newly diagnosed CML patients, and treatment of cells with antibodies blocking N-cadherin or H-cadherin reduces survival under conditions of direct contact.

Figure 4

a. Immunoblot analysis revealed restored β-catenin protein expression in shβcat-expressing CML CD34+ cells cultured in HS-5 DC (left, n=3). qRT-PCR confirmed β-catenin knockdown at the mRNA level (right, n=3). Immunoblots were normalized to α-tubulin and qRT-PCR was normalized to GUS. b. Consistent with restored β-catenin protein levels, HS-5 DC also restored colony forming ability of shβcat-expressing CML CD34+ cells under scenarios of DC in the presence of imatinib (n=4). c. Addition of an N-cadherin blocking antibody to co-cultures restored the effects of imatinib and shβcat on colony formation of CML CD34+ cells grown in HS-5 DC (n=3). d,e. Addition of antibodies blocking N-cadherin (n=9), H-cadherin (n=8), or both (n=6), to HS-5 co-cultures restored the effects of imatinib on CML CD34+ cells cultured under HS-5 DC in the presence of imatinib (d), but did not have an effect on β-catenin protein levels as demonstrated by immunoblot analyses (e). Bars represent SEM. *p<0.05.