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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Cell Biochem. 2016 Jan;117(1):118–125. doi: 10.1002/jcb.25255

Fig. 2. Real-time RT-PCR analysis of RANKL stimulated autophagosome associated gene expression in OSCC cells.

Fig. 2

The SCC14a cells were stimulated with different concentration of RANKL (0–100 ng/ml) for 48 h. Total RNA isolated from these cells was subjected to real-time RT-PCR analysis for LC3, Atg5, BECN-1 and PI3K-CIII mRNA expression using gene specific primers. The relative levels of mRNA expression were normalized with GAPDH amplification. Data shown are representative of three replicate studies. The values are expressed as mean ± SD (*p<0.05).