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. 2015 Dec 2;2(11):924–929. doi: 10.18632/oncoscience.275

Figure 1. Identification of differentially expressed miRNAs in HER2-overexpressed breast epithelial cells.

Figure 1

MCF10A-Vector and MCF10A-HER2 were used to screen for differentially regulated miRNAs. a) Western blot analysis of HER2-downstream signaling pathways in MCF10A-Vector and MCF10A-HER2 cells. b) Differentially expressed miRNAs in HER2-overexpressed cells. Over 300 miRNAs were screened using qPCR analysis. The differentially expressed miRNAs were selected with the 2-fold change cutoff. c) Agarose gel picture depicting the expression of representative qPCR products from the miRNA screening.