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. 2015 Jul 23;362(3):623–632. doi: 10.1007/s00441-015-2239-z

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© The Author(s) 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Multilineage differentation of rat ASC. (a) Representative images showing differentiation of ASC towards osteoblasts, shown by ALP staining, and adipocytes, shown by Oil Red O staining (a1-a4). No staining is found in the non-stimulated cells (a5-a8). (b) Representative images of differentiation towards cardiomyocytes, staining for connexin 43 (upper panel, b1-b4) and cTnI (lower panel, b5-b8) on cytospin slides. (c) Bar graph showing quantification of the number of positive ASC for cTnI (c1) and connexin 43 (c2) without (−) or stimulated (+) with 5-aza-2-deoxycytidin. No significant differences in differentiation between the three groups were found. Data shown as mean ± SD (Control and 7D group n = 6, 1D group n = 5)