Fig. 1.

Long (L) with-no-lysine kinase 1 (WNK1) expression increases high-conductance Ca²⁺-activated K⁺ (BK) α-subunit abundance. HEK293 cells were transfected with green fluorescent protein (GFP, control), BK α-subunit, or BK α-subunit and L-WNK1. A: L-WNK1 expression increases whole cell BK α-subunit abundance. Whole cell lysates were subjected to immunoblot with an anti-myc antibody to detect BK α-subunit, an anti-hemagglutinin (HA) antibody to detect L-WNK1, or an anti-actin antibody. B: summary of analyses of BK α-subunit whole cell expression. Immunoblots were quantified as described in materials and methods (n = 11, *P < 0.001) (Student's t-test). Results were normalized to BK α-subunit expression in the absence of exogenous L-WNK1.