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. 2015 Oct 14;310(1):F15–F26. doi: 10.1152/ajprenal.00226.2015

Fig. 4.

Fig. 4.

L-WNK1 increases whole cell BK expression in the absence of kinase activity. A: kinase dead L-WNK1 (K233M) increases whole cell BK α-subunit abundance. Whole cell lysates from HEK293 L-WNK1 KO cells transfected with GFP (control), BK α-subunit, or BK α-subunit with either wild-type L-WNK1 or L-WNK1 K233M were subjected to immunoblot with an anti-HA antibody to detect L-WNK1, an anti-myc antibody to detect BK α-subunit, or an anti-actin antibody. B: summary of analyses of BK α-subunit whole cell expression. Immunoblots were quantified as described in materials and methods (n = 8, *P < 0.01) (Student's t-test). Results were normalized to BK α-subunit expression in the absence of exogenous L-WNK1 or L-WNK1 K233M. Levels of expression of BK α-subunit were significantly increased by coexpression of L-WNK1 (P < 0.001) or by coexpression of the K233M mutant (P < 0.05) (Kruskal-Wallis test followed by Dunn's multiple-comparisons test).