Skip to main content
. 2015 Sep 16;310(1):F68–F84. doi: 10.1152/ajprenal.00205.2015

Fig. 5.

Fig. 5.

Colocalization of internalized eGFP-NPRA-WT and mutated motif FQQI/AAAA with early endosome antigen-1 (EEA-1) in MMCs. Colocalization experiments were performed 48 h after transfection of cells with eGFP-NPRA-WT and mutated motif FQQI/AAAA. Cells were treated with 100 nM ANP for different times. A: colocalization of eGFP-NPRA-WT and EEA-1 marker was observed after 5 and 10 min of treatment with ANP, after which the fluorescence intensity gradually decreased from 15 to 30 min. B: colocalization of mutant receptor FQQI/AAAA into endosomes. Colocalization of mutated eGFP-NPRA and EEA-1 marker was observed with a marked decrease by 57% compared with the WT receptor. C: quantification of the percent colocalization of eGFP-NPRA-WT and mutated motif FQQI/AAAA with EEA-1. The images shown are typical of the images obtained in 5 independent experiments. Values are means ± SE.