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. 2015 Sep 16;310(1):F68–F84. doi: 10.1152/ajprenal.00205.2015

Fig. 6.

Fig. 6.

Colocalization of internalized eGFP-NPRA mutated motif FQQI/AQQI or FQQI/AQQA with EEA-1 in cultured MMCs. Colocalization experiments were performed 48 h after transfection of cells with eGFP-NPRA mutated motifs FQQI/AQQI and FQQI/AQQA. Cells were treated with 100 nM ANP for different times. The mutation of FQQI/AQQA significantly decreased the receptor colocalization into endosomes. Colocalization of eGFP-NPRA mutated motif FQQI/AQQI with EEA-1 was decreased by 31% (A) and FQQI/AQQA was decreased by 55% (B) compared with the WT receptor. C: quantification of percent colocalization of eGFP-NPRA-WT, mutated motif FQQI/AQQI, and FQQI/AQQA with EEA-1. The images shown are typical of 4–5 independent experiments. Values are means ± SE.