Fig. 8.

Colocalization of internalized eGFP-NPRA-WT and mutated motif FQQI/AAAA with lysosome-associated membrane protein-1 (LAMP-1) in MMCs. Cells were treated with 100 nM ANP for different times. A: colocalization of eGFP-NPRA-WT with LAMP-1 marker was gradually increased from 5 to 30 min after ANP treatment. B: colocalization of eGFP-NPRA mutated motif FQQI/AAAA with LAMP-1 marker was decreased by 31%. C: quantification of the percent colocalization of eGFP-NPRA-WT and mutated motif FQQI/AAAA with LAMP-1. The images shown are typical of the images obtained in 4 independent experiments. Values are means ± SE.