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. 2015 Dec 1;109(11):2218–2226. doi: 10.1016/j.bpj.2015.10.014

Figure 2.

Figure 2

Verification of chromatin decompaction. (A) A representative image of CSK knockdown cell with nuclear and whole cellular boundary lineated. Scale bar, 2 μm. (B) Normalized image correlation function from nuclei (n = 18 cells for control, n = 20 cells treated). The gray area indicated the range of length scale where the difference between control and treated cells was significant by two sample t-tests (p < 0.05). Error bars indicate the mean ± SE. (C) Fitted DTEM values from C(r) in control and treated cells. (D) Magnified image of the nucleus after TOPRO 3 staining by confocal fluorescence microscopy. (E) The corresponding fluorescence intensity histogram from (D). The intensity standard deviation (std) indicates the heterogeneity of the chromatin. (F) Intensity standard deviation from cells treated with different concentrations of VPA for 24 h. ∗p < 0.05.