FIG. 7.

Male and female Gnaq^+/− mice exposed postnatally to TCDD have increased proportions of Th1 and Tregs. Spleen cells were isolated at 1 year of age from male and female Gnaq^+/− exposed to TCDD or vehicle peanut oil control (Vehicle) via lactation only, as in Figure 5. Cells were stained for flow cytometry and CD4⁺ T-cell populations were quantified as in Figure 4. A and E, The graphs depict the average number (± SEM) of CD4⁺ T cells in male (A) and female (E) Gnaq^+/− offspring. B–D and F–H, Representative flow plots show the proportion of CD4⁺ T-cell subsets in Gnaq^+/− mice lactationally exposed to vehicle (top row) and TCDD (bottom row), with the average percentage (± SEM) of each subset denoted on each plot. CD4⁺ T-cell populations in male mice are represented in B–D and female mice in F–H. Four to 11 Gnaq^+/− offspring from separately treated dams were used, and an * denotes a P value ≤ .05.