Figure 5.
Biofilms of S. mutans (A–C) and S. aureus (D–F) shown by conventional LIVE/DEAD kit staining (A, D), reflection from KMnO4 staining (B, E) and the combined images (C, F). Colocalization of green/red and blue channels (bacteria which were stained with LIVE/DEAD kit and also giving a strong reflected signal appeared light blue. In contrast with the P. aeruginosa biofilms, the EPS was more localized to the bacteria, however, in both biofilms there were some areas that had EPS, but were relatively devoid of bacteria (arrows). Scale bars = 50 μm.