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. 2015 Dec 11;6:287. doi: 10.3389/fphar.2015.00287

Figure 5.

Figure 5

TGR5 inhibits IκBα phosphorylation and p65 translocation in gastric cancer cells. (A) TGR5 overexpression with ligand treatment suppressed TNF-α-induced phosphorylation of IκBα in SGC7901 cells. Cells were transfected with TGR5 plasmid and then treated with ligand for 24 h. Finally, cells were treated with TNF-α for 1 h (n = 3) p-IκBα, phosphorylated IκBα; T-IκBα, total IκBα. (B) The data of relative protein levels in (A) are expressed as fold change over the ratio of p-IκBα to T-IκBα in the control group (lane 1). (C) TGR5 overexpression with ligand treatment suppressed p65 overexpression-induced the translocation of p65 in SGC7901 cells. Cells were cotransfected with TGR5 and/or p65 plasmids and then were treated with the ligand GPBARA for 24 h. (D) The data of relative protein levels in (C) are expressed as fold change over the ratio of p65 to Lamin B1 in the control group (lane 1). *P < 0.05.