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. 2015 Dec 1;109(11):2371–2381. doi: 10.1016/j.bpj.2015.10.023

Figure 2.

Figure 2

(A) Effect of temperature on the equilibrium constant KM for the formation of the MT1-MMP-substrate complex in the range 10–37°C, at atmospheric pressure in 50 mM Tris, 100 mM NaCl, and 5 mM CaCl2, at pH 7.4. The linear correlation coefficient is r = 0.953. (B) Michaelis-Menten plots for the determination of the steady-state kinetic parameters between 10 and 37°C.