Figure 8.

(A) Thermodynamic and kinetic parameters associated with the enzyme-substrate formation and catalysis (32). (B) Schematic energy diagram for the MT1-MMP-catalyzed hydrolysis reaction of a collagen-like peptide. The free energy ΔG°(1/K_m) and enthalpy ΔH°(1/K_m) change are associated with MT1-MMP-substrate formation. The activation free energy ΔG^‡(k_cat) and activation enthalpy ΔH^‡(k_cat) change correspond to the catalytic step of the enzymatic reaction. The resultant ΔG^‡_T(k_cat/k_M) and ΔH^‡_T(k_cat/k_M) are the net activation free energy and enthalpy difference for the E+S to ES^‡ transformation.