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. 2015 Nov 25;13(9):1765–1771. doi: 10.1016/j.celrep.2015.10.066

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Protaminized Somatic Nuclei Re-acquire a Nucleosome Organization after Nuclear Transfer

(A) Displacement Prm1 during pronucleus formation after nuclear transfer (NT). Scale bar represents 20 μm. Prm1 is red, and nucleus is blue.

(B) Incorporation TH2B in pronucleus (PN) after NT of protaminized nuclei: pronucleus (PN, blue), TH2B (green), Merge (TH2B/PN). Scale bar represents 20 μm.

(C) SCNT of fibroblast transfected with pPrm1-RFP. (a) Picture represents Prm1 (red)-positive fibroblasts used as donors for SCNT. (a1) Protaminized nucleus in injected capillary before nuclear transfer into enucleated MII sheep oocyte. (a2) In vitro development of nuclear transfer embryos.

(D) Table 2. Quality of blastocysts is as follows: IVF, in vitro fertilized; NT Prm1 cells. Total cell number is mean ± SD. Cellular composition is the number of inner cell mass/trophectoderm cell number. Karyotype composition is the cell with euploid chromosome number divided by total cells (%).