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. 2015 Dec 11;5:17935. doi: 10.1038/srep17935

Figure 4. Genipin inhibits UCP2 expression and induces ROS accumulation.

Figure 4

(A) LPS-primed BMDMs were pretreated with genipin and then stimulated with ATP or Salmonella. The cells were fixed, permeabilized and stained for UCP2 (green). DAPI was used to label nuclei (blue). (B) LPS-primed BMDMs stimulated with genipin and/or Salmonella were lysed and blotted for UCP2. (C) LPS-primed BMDMs were stimulated with genipin and/or ATP and/or Salmonella. The cells were incubated with MitoSox Red for 10 min and analyzed using a FACSAria flow cytometer. (D) MitoSox MFI was analyzed using FCS express. (E) LPS-primed BMDMs were pretreated with genipin and/or NAC at the indicated doses and then stimulated with nigericin. IL-1β release in the cell supernatants was determined by ELISA. The data are representative of three independent experiments. **P < 0.01.