Figure 3. C/EBPβ promotes the transcription of the porcine DKK2 gene.

(A) Overexpression of C/EBPβ stimulated DKK2 mRNA expression in PK cells. (B) C/EBPβ was transfected into PK cells, and transfection efficiency was determined using Western blot analysis. (C) Knockdown of C/EBPβ was confirmed using qRT-PCR analysis. PK cells were stimulated using siRNA or NC (10 μl) for 24 h. (D) The expression of the DKK2 protein was determined using Western blot analysis. **P < 0.01.