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. 2015 Dec 11;5:17162. doi: 10.1038/srep17162

Figure 3. Levels of pAKT and pPRAS40 were reduced within 2 hours of treating with ARQ 092 and remained low for 72 hours.

Figure 3

Mutation-positive (clone 1) and mutation-negative (clone 2) SCCs were cultured in the presence or absence of serum with 125 nM ARQ 092 and lysed at the times indicated. The media was not changed during the course of the experiment. (a) Infrared images of western hybridizations using the indicated antibodies. (b) Histograms of the ratios of the infrared signals for each antibody pair. The inset in the pAKT histogram shows the ratios from the ARQ 092-treated cells using a smaller scale. Note the increased signal from CL1 lysates grown in serum-free medium at 8, 48 and 72 hours. Results of additional experiments are shown in Supplementary Fig. 4. CL1, clone 1 (blue bars); CL2, clone 2 (red bars), ser, grown with serum (solid bars); NS, grown without serum (shaded bars).