Figure 4.

Reduced BDNF-6 dendritic targeting in BDNF^Met/Met mice after pilocarpine treatment. High-magnification pictures of the in situ hybridization and densitometric analysis of dendrites at progressive distances from soma for: (A) BDNF2-mRNA, (B) BDNF-4, and (C) BDNF-6 mRNAs in mouse hippocampal CA1, CA3, and dental gyrate (DG) regions of BDNF^Val/Val and BDNF^Met/Met mice. Data are expressed by the gray level of the staining; the distance is expressed in µm from the point of emergence of the apical dendrites from the soma (=0 µm), and the bar represents the mean ± standard error of the mean (n= 5 independent biological replicates). *p < 0.05; **p < 0.01. d.rad, distal radiatum; IML, inner molecular layer; lac.mol, lacunosum molecular layer; MML, medial molecular layer; OML, outer molecular layer; p.rad, proximal radiatum; s.gr, stratum granularis; s.luc, stratum lucidum; s.pyr, stratum pyramidale.