Table 1.
List and Sequence of Primers Used in This Study
| Gene | Forward | Reverse |
|---|---|---|
| Real time PCR | ||
| BDNF-1 a | CCTGCATCTGTTGGGGAGAC | CGCCTTCATGCAACCGAAGTAT |
| BDNF-2 a | ACCTTTTCCTCCTCCTGCG | TGGATGAAGTACTACCACCTCGG |
| BDNF-3 a | TGAGACTGCGCTCCACTCCC | CGCCTTCATGCAACCGAAGTAT |
| BDNF-4 a | CAGAGCAGCTGCCTTGATGTTT | CGCCTTCATGCAACCGAAGTAT |
| BDNF-6 a | ACAATGTGACTCCACTGCCGG | CGCCTTCATGCAACCGAAGTAT |
| BDNF-7 a | ACTTACAGGTCCAAGGTCAACG | GGACAGAGGGTCGGATACAG |
| BDNF-8 a | ATGACTGTGCATCCCAGGAGAAA | CGCCTTCATGCAACCGAAGTAT |
| Total BDNF a | TCGTTCCTTTCGAGTTAGCC | TTGGTAAACGGCACAAAAC |
| EZH2 a | CAACCCTGTGACCATCCACG | CGACATCCAGGAAAGCGGTT |
| ß-actin a | GCCAGAGCAGTAATCTCCTTCT | AGTGTGACGTTGACATCCGTA |
| Gapdh a | CGTGCCGCCTGGAGAAACC | TGGAAGAGTGGGAGTTGCTGTTG |
| ChIP | ||
| pBDNF-1 b | TGATCATCACTCACGACCACG | CAGCCTCTCTGAGCCAGTTACG |
| pBDNF-2 b | CCGTCTTGTATTCCATCCTTTG | CCCAACTCCACCACTATCCTC |
| pBDNF-3 b | GTGAGAACCTGGGGCAAATC | ACGGAAAAGAGGGAGGGAAA |
| pBDNF-4 b | CTTCTGTGTGCGTGAATTTGCT | AGTCCACGAGAGGGCTCCA |
| pBDNF-5 | GCTGAAGGCGTGCGAGTATT | GGCCGATATGTACTCCTGTTCTG |
| pBDNF-6 b | ACTCACACTCGCTTCCTCCT | GCACTGGCTTCTCTCCATTT |
| pBDNF-7 | GGAAGGGTGGTGAGAGAGATATAGAG | GTGCCTAAGCCGGTGGTAAG |
| pBDNF-8 | TGAAAGGAAAAAGGAGAGGCTTT | AGAAAATGCCACTCCCACATAAG |
aPrimer sequences as in Ieraci et al. (2015).
bPrimer sequences as in Tsankova et al. (2006).
BDNF, brain-derived neurotrophic factor; ChIP, chromatin immunoprecipitation; EZH2, enhancer of zeste homolog 2; Gapdh, glyceraldehyde-3-phosphate dehydrogenase; PCR, polymerase chain reaction.