Figure 1. Schematic illustration of the screening platform.

(1) CHO cells are co-transfected with plasmids encoding target genes and plasmids encoding S11(Split-GFP)-tagged model protein. (2) Transfected cells are cultivated in a 96-half-deepwell microplate capped with a low-evaporation Duetz sandwich cover (cover not shown). (3) Viable cell density is determined using image fluorescence cytometry on Hoechst and propidium iodide (PI) stained cells. (4) Upon addition of GFP1–10_OPT to supernatants, relative product titers of supernatants are determined by split-GFP complementation.