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. 2015 Dec 11;5:18016. doi: 10.1038/srep18016

Figure 5. Co-expression of target genes affects split-GFP-based specific and volumetric productivity of hα1AT-S11 and hC1INH-S11.

Figure 5

(a,b) Plasmid encoding hα1AT N-terminally tagged with S11_M3 was co-transfected with mock plasmid or plasmids encoding target (mouse) genes into CHO-S cells in 96-HDW-microplates. Three days after transfection, supernatants were obtained and subjected to split-GFP product titer assay for determination of relative titer. Split-GFP-based specific productivity (qp) and titer are shown. Eight separate transfections were performed in each experiment and experiments were performed twice (n = 2; 16 wells). Mean and standard deviation are depicted. Only statistically significant (p ≤ 0.05) increases in productivity are shown. (c,d) As described for panels (a,b), with the only difference being use of plasmid encoding hC1INH C-terminally tagged with S11_M3 instead of plasmid encoding hα1AT. (e,f) Comparison of split-GFP-based product titer assay and ELISA. hα1AT (mock and Atf4 ) and hC1INH (Mock and Xbp1s) supernatants described in panels a-d were subjected to ELISA for determination of hα1AT and hC1INH titers. Mean, standard deviation and percentage difference are shown.