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. 2015 Dec 11;5:18118. doi: 10.1038/srep18118

Figure 6. TGFBR1 negatively regulated adipogenesis.

Figure 6

The mRNA expression pattern of Tgfbr1 was examined in ST2 during differentiation (a). The Tgfbr1 expression plasmid overexpresses Tgfbr1 mRNA in ST2 cells (b). Overexpression of Tgfbr1 in ST2 decreased adipocyte formation (c,d). The mRNA and protein levels of PPARγ, C/EBPα, aP2 and adipsin were inhibited (e,h). In contrast, transfection of the two Tgfbr1 siRNAs both efficiently knockdown mRNA level of Tgfbr1 (g), promoted adipocyte differentiation (h,i), and induced the mRNA and protein levels of PPARγ, C/EBPα, aP2 and adipsin in ST2 cells (j,k). Values represent the mean ± SD of three experiments. (a) *Significant vs. -2 day, p < 0.05, #Significant vs. 1 day, p < 0.05; (b–j) *Significant vs. control transfection, p < 0.05.