Figure 3. Inhibition of LdNAT1 expression by 20-hydroxyecdysone (20E) signaling in Leptinotarsa decemlineata.
The newly-ecdysed third-instar larvae have ingested potato foliage treated with water (control), 0.1 μg/mL halofenozide or 20E for 1 day (A). Otherwise, the newly-ecdysed third-instar larvae have ingested PBS-, dsegfp-, or dsSHD-dipped leaves (B–D); or PBS-, dsegfp-, or dsE75-dipped leaves for 3 days (E–G); or PBS-, dsegfp-, or dsFTZ-F1-dipped leaves (H–J) for 3 days. Different letters indicate significant difference at P value < 0.05.