Table 2. The contents of seven amino acids in the bodies and feces of the Leptinotarsa decemlineata larvae fed on PBS, dsegfp, dsNAT1-1 and dsNAT1-2-immersed foliage for 3 days.
| Amino acid | Origin | Concentration (μmol/g) |
|||
|---|---|---|---|---|---|
| PBS | dsegfp | dsNAT1-1 | dsNAT1-2 | ||
| The third-instar larvae | |||||
| C | Bodies | 11.12 ± 0.51 a | 13.12 ± 0.84 a | 3.21 ± 0.21 b | 2.67 ± 0.14 b |
| Feces | 0.25 ± 0.02 b | 0.33 ± 0.03 b | 2.34 ± 0.14 a | 3.15 ± 0.13 a | |
| H | Bodies | 20.88 ± 1.24 a | 20.02 ± 1.11 a | 10.70 ± 0.52 b | 11.17 ± 0.47 b |
| Feces | 4.69 ± 0.20 b | 4.06 ± 0.28 b | 8.84 ± 0.41 a | 7.87 ± 0.52 a | |
| I | Bodies | 41.49 ± 2.22 a | 42.16 ± 3.04 a | 26.77 ± 1.29 b | 28.13 ± 1.38 b |
| Feces | 15.41 ± 0.84 b | 12.68 ± 0.61 b | 27.22 ± 1.15 a | 29.39 ± 1.74 a | |
| L | Bodies | 58.48 ± 2.41 a | 58.76 ± 1.98 a | 32.45 ± 2.07 b | 34.68 ± 2.15 b |
| Feces | 20.31 ± 1.06 b | 19.75 ± 1.19 b | 37.55 ± 1.24 a | 33.17 ± 2.03 a | |
| M | Bodies | 1.74 ± 0.38 a | 4.26 ± 0.25 a | 0.64 ± 0.04 b | 0.13 ± 0.01 b |
| Feces | 0.02 ± 0.00 b | 0.03 ± 0.00 b | 0.31 ± 0.02 a | 0.25 ± 0.02 a | |
| F | Bodies | 27.55 ± 1.07 a | 28.64 ± 1.13 a | 19.20 ± 1.23 b | 15.73 ± 1.19 b |
| Feces | 11.56 ± 0.81 b | 9.62 ± 0.57 b | 23.69 ± 1.04 a | 18.96 ± 1.07 a | |
| S | Bodies | 55.50 ± 2.11 a | 55.86 ± 4.08 a | 41.88 ± 2.58 b | 34.26 ± 2.14 b |
| Feces | 13.27 ± 1.01 b | 11.67 ± 0.72 b | 31.84 ± 1.22 a | 29.62 ± 1.57 a | |
| The fourth-instar larvae | |||||
| C | Bodies | 6.89 ± 0.51 a | 10.50 ± 0.67 a | 2.10 ± 0.16 b | 0.25 ± 0.02 c |
| Feces | 0.25 ± 0.02 b | 0.25 ± 0.02 b | 4.58 ± 0.37 a | 7.70 ± 0.51 a | |
| H | Bodies | 21.32 ± 0.71 a | 19.86 ± 0.54 a | 9.44 ± 0.35 b | 10.50 ± 0.49 b |
| Feces | 4.69 ± 0.57 b | 4.66 ± 0.41 b | 8.64 ± 0.37 a | 12.06 ± 0.61 a | |
| I | Bodies | 39.21 ± 1.13 a | 45.54 ± 2.02 a | 21.79 ± 1.51 b | 20.38 ± 1.44 b |
| Feces | 15.40 ± 0.87 b | 13.72 ± 0.74 b | 30.73 ± 1.57 a | 27.28 ± 1.63 a | |
| L | Bodies | 65.00 ± 3.54 a | 63.12 ± 4.13 a | 38.40 ± 2.33 b | 37.77 ± 1.79 b |
| Feces | 23.30 ± 1.47 b | 26.91 ± 1.56 b | 46.79 ± 3.64 a | 48.52 ± 4.11 a | |
| M | Bodies | 1.60 ± 0.11 a | 1.54 ± 0.08 a | 0.65 ± 0.02 b | 0.41 ± 0.01 b |
| Feces | 0.15 ± 0.01 b | 0.40 ± 0.02 b | 2.64 ± 0.15 a | 1.51 ± 0.11 a | |
| F | Bodies | 26.25 ± 1.47 a | 29.72 ± 1.55 a | 11.51 ± 0.71 b | 14.34 ± 0.57 b |
| Feces | 11.55 ± 0.57 b | 12.97 ± 0.69 b | 22.34 ± 1.21 a | 24.58 ± 1.54 a | |
| S | Bodies | 52.88 ± 3.74 a | 59.52 ± 3.69 a | 26.64 ± 1.27 b | 28.63 ± 1.34 b |
| Feces | 16.27 ± 0.79 b | 15.34 ± 0.85 b | 31.11 ± 1.45 a | 42.19 ± 1.85 a | |
Free amino acid contents in the bodies and feces are analyzed with a Beckman 6300 Amino Acid Analyzer. Difference of amino acid content within each line is analyzed by ANOVA followed by the Tukey-Kramer test. Data that do not share the same letters are significantly different at P values of 0.05.