Table 3. The developing stage of L. decemlineata surviving larvae subjected to dietary dsRNA introduction.
| Larval instar | 2nd | 3rd | 4th | Prepupae | |
|---|---|---|---|---|---|
| Initiation of the bioassay at the early second instar stage | |||||
| CK | 2.0 ± 0.1 a | 2.1 ± 0.2 a | 4.1 ± 0.2 a | 5.8 ± 0.3 a | 14.0 ± 0.4 a |
| dsegfp | 2.2 ± 0.2 a | 2.0 ± 0.1 a | 4.0 ± 0.2 a | 5.6 ± 0.3 a | 13.8 ± 0.5 a |
| dsNAT1-1 | 2.1 ± 0.1 a | 2.1 ± 0.2 a | 5.1 ± 0.2 b | 6.7 ± 0.4 b | 16.0 ± 0.6 b |
| dsNAT1-2 | 2.1 ± 0.1 a | 2.2 ± 0.2 a | 5.2 ± 0.3 b | 6.8 ± 0.4 b | 16.3 ± 0.5 b |
| Initiation of the bioassay at the early third instar stage | |||||
| CK | 2.0 ± 0.1 a | 4.0 ± 0.2 a | 6.0 ± 0.2 a | 12.0 ± 0.3 a | |
| dsegfp | 2.1 ± 0.2 a | 4.2 ± 0.1 a | 5.8 ± 0.4 a | 12.1 ± 0.4 a | |
| dsNAT1-1 | 2.0 ± 0.1 a | 5.4 ± 0.2 b | 7.6 ± 0.3 b | 15.0 ± 0.5 b | |
| dsNAT1-2 | 2.2 ± 0.1 a | 5.3 ± 0.2 b | 7.5 ± 0.3 b | 15.0 ± 0.4 b | |
The larval growth is checked at 4-hr intervals. See text for detail explanation for the identification of instars and prepupae. The data are given as means ± SE, and are subjected one-way ANOVA and followed by the Tukey–Kramer test. Means on the same column followed by the same letters are not significantly different at P < 0.05.