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. Author manuscript; available in PMC: 2015 Dec 11.
Published in final edited form as: Biochemistry. 2010 Aug 3;49(30):6451–6461. doi: 10.1021/bi100769k

Table 3.

Rate Constants for Interactions of Mutant APE1 with AP- and F-Containing DNA

rate constants mutant APE1/AP-substrate mutant APE1/F-substrate
k1 (M −1 s −1) (1.78 ± 0.07) × 103 (5.4 ± 0.2) × 103
k−1 (s −1) (1.51 ± 0.05) × 10−3 (2.1 ± 0.1) × 10−3
k2 (s −1) (2.2 ± 0.1) × 10−4 (1.5 ± 0.01) × 10−4
k−2 (s −1) (5.4 ± 0.2) × 10−3 (1.12 ± 0.01) × 10−3
Ka (M−1) (4.81 ± 0.02) × 104 (3.44 ± 0.01) × 105
Ktitr (M) (2.9 ± 0.4) × 10−6