Fig. 7. Correlation of transcriptomic and proteomic analyses of Nrf2 activity in the mouse kidney.
Microarray and iTRAQ analyses were performed on kidney tissue from Nrf2+/+ or Nrf2−/− mice treated with vehicle (DMSO) or CDDO-Me for 24 h. DE; differentially expressed. The relevant tables are indicated in the boxes. From the microarray analysis, 12 genes were shown to be both significantly downregulated in the kidneys of Nrf2−/− mice and significantly upregulated in the kidneys of Nrf2+/+ mice treated with CDDO-Me. From the iTRAQ analysis, 9 of the genes were also shown to be significantly downregulated at the protein level in the kidneys of Nrf2−/− mice (the remaining 3 genes were not detected at the protein level by iTRAQ). These 9 genes/proteins are therefore considered to be most sensitive to genetic inhibition and pharmacological induction of Nrf2 in the mouse kidney.