Table 1. Genes/proteins sensitive to genetic inhibition and pharmacological induction of Nrf2 in the mouse kidney.

Microarray and iTRAQ analyses were performed on kidney tissue from Nrf2^+/+ or Nrf2^−/− mice treated with vehicle (DMSO) or CDDO-Me for 24 h. The 12 genes shown above were shown to be both significantly downregulated in the kidneys of Nrf2^−/− mice and significantly upregulated in the kidneys of Nrf2^+/+ mice treated with CDDO-Me. From the iTRAQ analysis, 9 of the genes were also shown to be significantly downregulated at the protein level in the kidneys of Nrf2^−/− mice. The remaining 3 genes were not detected (N.D.) at the protein level by iTRAQ. These 9 genes/proteins are therefore considered to be most sensitive to genetic inhibition and pharmacological induction of Nrf2 in the mouse kidney.

			Microarray					iTRAQ
			Nrf2−/− vs. Nrf2+/+		CDDO-Me vs. Vehicle			Nrf2−/− vs. Nrf2+/+		CDDO-Me vs. Vehicle
Gene Symbol	David ID	RefSeq	Ratio	Adj. P-value	Ratio	Adj. P-value	Accession	Ratio	P-value	Ratio	P-value
Abcc2	451709	NM_013806	0.77	9.43−03	1.46	2.79−04	Q8VI47	0.79	6.66−03	1.22	4.76−03
Aldh1l1	430403	NM_027406	0.72	8.21−03	1.38	9.42−03	Q8R0Y6	0.33	5.14−10	1.08	2.12−01
Cyp2a5	420632	NM_007812	0.27	6.17−05	2.04	2.01−02	P20852	N.D.	N.D.	N.D.	N.D.
Dpys	425658	NM_001164466	0.29	8.53−06	1.67	4.28−02	Q9EQF5	0.66	3.78−02	1.04	7.75−01
Gclm	425831	NM_008129	0.76	1.40−03	1.28	2.96−03	O09172	0.81	2.06−02	1.05	5.59−01
Gsta3	423583	NM_001077353	0.27	8.20−06	2.13	2.89−03	P30115	0.16	8.62−08	0.90	3.42−01
Gstm1	443311	NM_010358	0.38	3.81−06	1.90	4.37−04	P10649	0.09	1.14−10	1.16	5.82−02
Nampt	465583	NM_021524	0.70	1.08−02	1.44	1.07−02	Q99KQ4	0.61	8.19−03	1.22	1.14−01
Nqo1	481143	NM_008706	0.29	1.45−04	3.47	1.39−04	Q64669	0.28	1.66−07	1.36	1.02−03
Slc35e3	443078	NM_029875	0.84	3.44−02	1.22	1.96−02	Q6PGC7	N.D.	N.D.	N.D.	N.D.
Spatc1	429270	NM_028852	0.63	1.68−02	1.80	2.38−03	Q148B6	N.D.	N.D.	N.D.	N.D.
Ugt1a10	446962	NM_201641	0.58	9.04−04	1.45	2.12−02	Q6ZQM8	0.25	6.18−05	1.09	5.50−01