Fig. 1.

Schematic diagrams of the recombinant virus PRV TK⁻/gE⁻/PrM-E⁺ and transfer vactor. a The illustration of the genomic map of the parental attenuated PRV TK⁻/gE⁻/LacZ⁺ in which a LacZ expression cassette was inserted into the coding region of gE gene. The PRV genomic DNA is approximately 150 kb including a unique longe region (UL), a unique short region (Us), internal repeat (IR) and terminal repeat (TR). ∆TK represent the deleted thymidine kinase gene. b The map of transfer plasmid pIE-CAG-PrM-E-BGH in which partial coding region of gE and gI were replaced by a expression cassette containing the sequence of human cytomegalovirus (hCMV) enhancer, chicken β-actin promoter (CAG), PrM-E gene of JEV SX09S-01 strain, BHG poly-A signal (BHG pA). The PRV 28 k, 11 k, gE and gI partial and gD gene of PRV in the transfer plasmid to facilitate homologous. c Genome of recombinant PRV TK⁻/gE⁻/PrM-E⁺ virus, in which partial gI and gE gene are replaced by a PrM-E gene expression cassette. PRV TK⁻/gE⁻/PrM-E⁺ was constructed by homologous recombination on PK-15 cells between the genome of parental PRV TK⁻/gE⁻/LacZ⁺ and the transfer plasmid pIE-CAG-PrM-E-BGH