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. 2015 Dec 10;12:214. doi: 10.1186/s12985-015-0449-3

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© Qian et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Humoral immune responses against PRV in immunized mice. Serum samples were collected at weekly intervals after immunization. a Serum samples of PRV-specific antibodies using the PRV-gB Antibody Test Kit by indirect ELISA, according to the manufacturer’s instructions. b PRV Neutralizing antibodies were measured in serum samples at different times by serum neutralization test. PRV Neutralizing antibodies were calculated and expressed as the reciprocal of the highest serum dilution that inhibits 50 % of the culture wells of the PRV replication. Data represent the mean ± SEM. Statistical significance was assessed by Student’s t-test (***: P < 0.001). Data represent the mean ± SEM. Different letters (a, b, c) indicate a statistically significant difference between the different experimental groups (P < 0.05)