Table 2.

The plaque numbers of serum samples in immunized mice by plaque reduction neutralization test (PRNT) assay

Dilution of Seruma	Numbers of Plaque by PRNT
	DMEM	PRV TK−/gE−/LacZ+	PRV TK−/gE−/PrM-E+	JEV Vaccine
1:4	64.5 ± 0.5	59.5 ± 1.5	23.5 ± 1.5	11.0 ± 1.0
1:8	62.5 ± 2.5	57.5 ± 2.5	35.0 ± 1.0	19.5 ± 0.5
1:16	63.5 ± 1.5	60.5 ± 0.5	55.5 ± 0.5	24.0 ± 1.0
1:32	62.5 ± 0.5	63.5 ± 1.5	60.5 ± 1.5	34.0 ± 1.5
1:64	63.0 ± 1.5	62.5 ± 0.5	63.5 ± 0.5	62.5 ± 2.5

^aThe sera of mice at 8 weeks post immunization was diluted and mixted with 200PFU of JEV SX/09/01 strain for 1 h at 37 °C, and then added to BHK-21 in 24-well plate by PRNT assay