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. 2015 Dec 10;15:114. doi: 10.1186/s12935-015-0265-2

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© Liu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — ESC reversed EMT induced by TGF-β in HepG2 cells by down-regulation of Vimentin and up-regulation of E-cadherin. a Expressions of Vimentin and E-cadherin in HepG2 cells pretreated with different concentrations (1–5 μg/mL) of ESC prior 2 h to TGF-β (5 ng/mL) incubation for 24 h with immune-fluorescence assay. The magnification is ×400. b Western blot results of Vimentin and E-cadherin in HepG2 cells pretreated with different concentrations (0.2–5 μg/mL) of ESC prior 2 h to TGF-β (5 ng/mL) incubation for 24 h. c Western blot results of Vimentin and E-cadherin in HepG2 cells pretreated with 5 μg/mL ESC for different hours (1–4 h) prior to 5 ng/mL TGF-β stimulation. Western blot data presented were representative of those obtained in at least 3 separate experiments. The value of the control cells was set to 1