Table 2.
Purification of extracellular MnP from G. lucidum IBL-05
| Procedure | Total volume (mL) | Total protein (mg) | Total activity (UI)a | Specific activityb (UI mg−1) | Purificationc (fold) |
|---|---|---|---|---|---|
| Crude extract | 300 | 1302.3 | 215220 | 165.3 | 1 |
| Ammonium Sulphate ppt. | 22 | 43.9 | 13501.4 | 307.5 | 1.86 |
| Dialysis | 21 | 39.2 | 12559.9 | 320.4 | 1.94 |
| DEAE-cellulose | 12 | 6.37 | 6811.2 | 1069.3 | 6.47 |
| Sephadex G-100 | 9 | 2.9 | 4599.1 | 1585.9 | 9.6 |
aUI is defined as the amount of enzyme converting 1 μmol of substrate per minute.
bSpecific activity = UI per mg protein.
cPurification fold= Specific activity at given step/Specific activity of initial extract.