Figure 7.

Ponto-cerebellar mossy fiber axons are stable in control, enriched, and deprived conditions. Mossy fiber axons were manually traced for each imaging time point. Tracings were used to quantify the morphological parameters of individual mossy fiber axons across time. For the purposes of displaying data, time points were aligned by collecting data into 6 d time bins. Day 0 is the day animals were introduced into sensory enriched or deprived housing conditions. For the control group, day 0 was taken as the approximate midpoint of imaging. Data were normalized and are reported as the percentage of day 0. Data are presented as the mean ± SEM. Total axon length shows the mean percentage change in the total length of individual axons. The number of branch points refers to the total number of branch points per axon. Branch length was measured for individual axonal branches. Interterminal path length refers to the axonal path length separating two terminals. Tortuosity was calculated as the ratio of the Euclidian distance between two terminal points and the axonal path length between those points of individual axons. The term “convex hull volume” refers to the convex hull volume of individual axons.