TABLE 2 .
Antibiotic susceptibilities of SCVs in comparison to their parental B. cereus wild-type strains as assessed by the CLSI disk diffusion assay
| Antibiotica | Susceptibility of wild-type strainb |
Susceptibility of SCVb |
||||||
|---|---|---|---|---|---|---|---|---|
| F3351/87 | RIVM Bc51 | UHDAM 143/pl | F3351/87/SCV/AN | F3351/87/SCV/GM | RIVM Bc51/SCV/AN | UHDAM 143/pl/SCV/AN | UHDAM 143/pl/SCV/GM | |
| Amikacin | S (26) | S (26) | S (26) | I (18) | I (19) | I (17) | I (17) | I (17) |
| Amoxicillin/clavulanic acid | R (15) | R (16) | R (16) | S (20) | S (17) | S (18) | S (19) | S (19) |
| Azithromycin | S (22) | S (21) | S (22) | S (24) | S (23) | S (23) | S (25) | S (24) |
| Cefepime | R (10) | R (11) | R (9) | S (22) | S (19) | S (23) | S (22) | S (21) |
| Chloramphenicol | S (27) | S (27) | S (27) | S (28) | S (26) | S (28) | S (29) | S (27) |
| Ciprofloxacin | S (26) | S (26) | S (28) | S (29) | S (28) | S (29) | S (31) | S (32) |
| Clindamycin | S (22) | S (21) | S (22) | S (20) | S (20) | S (19) | S (21) | S (19) |
| Colistin | R (0) | R (0) | R (0) | R (0) | R (0) | R (0) | R (0) | R (0) |
| Enrofloxacin | S (26) | S (26) | S (28) | S (26) | S (26) | S (26) | S (28) | S (28) |
| Erythromycin | S (27) | S (27) | S (27) | S (29) | S (28) | S (28) | S (30) | S (29) |
| Fusidic acid | R (12) | R (12) | R (13) | I (17) | I (16) | I (17) | I (16) | I (16) |
| Gentamicin | S (26) | S (23) | S (23) | S (16) | S (16) | S (16) | S (16) | S (16) |
| Imipenem | S (38) | S (36) | S (40) | S (38) | S (36) | S (37) | S (39) | S (37) |
| Linezolid | S (29) | S (29) | S (30) | S (32) | S (30) | S (31) | S (31) | S (30) |
| Meropenem | S (31) | S (32) | S (33) | S (32) | S (30) | S (33) | S (33) | S (33) |
| Mupirocin | R (0) | R (0) | R (0) | R (8) | R (9) | R (9) | R (9) | R (9) |
| Penicillin | R (11) | R (12) | R (13) | R (12) | R (12) | R (12) | R (12) | R (13) |
| Rifampin | I (18) | I (17) | I (19) | S (20) | S (21) | S (20) | S (20) | I (19) |
| Sulfonamide/trimethoprim | R (0) | R (6) | R (0) | R (0) | R (0) | R (0) | R (0) | R (0) |
| Teicoplanin | S (19) | S (18) | S (19) | S (19) | S (18) | S (20) | S (20) | S (19) |
| Tetracycline | S (23) | S (23) | S (24) | S (25) | S (26) | S (25) | S (26) | S (26) |
| Tigecycline | S (23) | S (23) | S (24) | S (26) | S (25) | S (26) | S (27) | S (26) |
| Vancomycin | S (20) | S (20) | S (20) | S (22) | S (20) | S (21) | S (22) | S (21) |
For details on antibiotic concentrations, see Table S1 in the supplemental material.
For strain details, see Table 1. Results printed in boldface either indicate increased resistance of the SCV versus the wild type toward the tested antibiotic (visible by a reduction of the disk diffusion zone of ≥3 mm) or indicate enhanced sensitivity toward the respective antibiotic (visible by an enlargement of the disk diffusion zone of ≥3 mm). The letters S, I, and R indicate the sensitivity breakpoints as given by the CLSI/EUCAST (see Materials and Methods for details): S, sensitive; R, resistant, I, intermediate susceptibility toward the antibiotic. The numbers in parentheses indicate the diameter (in millimeters) of the zone of inhibition on Mueller-Hinton agar.