Skip to main content
. 2015 Apr 22;63(10):1738–1752. doi: 10.1002/glia.22841

Figure 2.

Figure 2

Time‐dependent regulation of Sbno2 gene expression by hyper‐IL‐6 in murine and human astrocytes. Primary murine astrocytes (A–C) or primary human astrocytes (D) were treated with or without 25 ng/mL hyper‐IL‐6. Cells were harvested at the indicated time points and total RNA was isolated and analyzed by RPA (A–C) or qPCR (D). A: A representative RPA is shown. Graphs show mean ± SEM of integrated density of Sbno2 (B) or Socs3 (C) mRNA bands relative to the control RPL32 of two independent RPAs, each 2–3 samples per group (total n = 5–6). *P < 0.05, **P < 0.01, ***P < 0.001 compared by ANOVA and Tukey's post test. D: The graph shows means ± SEM of three independent qPCR runs with each 1–3 samples per group (total n = 7). Values are Sbno2 mRNA copy numbers per 106 18S (internal control). *P < 0.05, **P < 0.01, ***P < 0.001 compared by ANOVA and Tukey's post test.